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NASC ID: N534088

Name: SALK_034088

ABRC stock number: SALK_034088

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_034088.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G18660.3=P355 and AT3G18670.1 (at chr 3 pos 6423801 (W/6423738-6423801) on the TAIR10). The sequences' 1-65 bps mapped on the genome, 60-78 bps onto the T-DNA. Pools P 8, R 1, C 4 N 9. The insertion site is at the 3' end of the sequence mapping. Genbank Acc KO384554.

SALKseq_034088.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G63410.1 (at chr 3 pos 23415850 (W/23415812-23415850) on the TAIR10). The sequences' 1-39 bps mapped on the genome, 46-78 bps onto the T-DNA. Pools P 8, R 1, C 4 N 9. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO381202.

SALKseq_034088.101 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G19560.1 and AT3G19570.2=P355 (at chr 3 pos 6796833 (W/6796780-6796833) on the TAIR10). The sequences' 1-54 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 8, R 1, C 4 N 9. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO387901.

SALKseq_034088.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 3' of AT5G47060.1 (at chr 5 pos 19117924 (C/19117924-19118001) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 8, R 1, C 4 N 9. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO391244.

Donation Date: 2002-06-26

Donated by:  The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_034088

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_034088C.

Status Price (£)
Available £10.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene: Allele: Locus: AGI code:

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation - At3g18670

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.