NASC Stock Detail Page
NASC ID: N534809
Name: SALK_034809
ABRC stock number: SALK_034809
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. Associated Polymorphisms: SALKseq_034809.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G36810.1 and AT2G36815.1 (at chr 2 pos 15440727 (W/15440688-15440727) on the TAIR10). The sequences' 1-40 bps mapped on the genome, 40-79 bps onto the T-DNA. Pools P 3, R 4, C 6 N 5. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO391278. SALKseq_034809.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G59790.1 and AT1G59800.1 (at chr 1 pos 22004902 (C/22004902-22004957) on the TAIR10). The sequences' 1-56 bps mapped on the genome, 60-79 bps onto the T-DNA. Pools P 3, R 4, C 6 N 5. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO377896. SALKseq_034809.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G64250.1 (around chr 1 pos 23841334 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc KO414687. SALKseq_034809.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G64270.1 (at chr 1 pos 23851269 (C/23851269-23851309) on the TAIR10). The sequences' 1-41 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 3, R 4, C 6 N 5. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO384589. SALKseq_034809.4 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 3' of AT1G26580.1 (at chr 1 pos 9187463 (W/9187410-9187463) on the TAIR10). The sequences' 1-54 bps mapped on the genome, 59-79 bps onto the T-DNA. Pools P 3, R 4, C 6 N 5. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO387936.
Donation Date: 2001-01-12
| Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_034809
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: Homozygous/confirmed line: SALK_034809C.
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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Phenotype
Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation - At2g36820
References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.