NASC

NASC ID: N536985

Name: SALK_036985

ABRC stock number: SALK_036985

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_036985.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 3' of AT3G06750.1 (at chr 3 pos 2129703 (W/2129630-2129703) on the TAIR10). The sequences' 1-74 bps mapped on the genome. Pools P 11, R 13, C 19 N 14. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO354553.

SALKseq_036985.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G48630.1=P355 and AT3G48640.1 (at chr 3 pos 18020810 (C/18020810-18020852) on the TAIR10). The sequences' 1-43 bps mapped on the genome, 58-79 bps onto the T-DNA. Pools P 11, R 13, C 19 N 14. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO351216.

SALKseq_036985.101 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G21950.1 and AT4G21960.1=UTR3 (at chr 4 pos 11644989 (W/11644928-11644989) on the TAIR10). The sequences' 1-62 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 11, R 13, C 19 N 14. The insertion site is at the 3' end of the sequence mapping. Genbank Acc KO357885.

SALKseq_036985.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G23150.1 (at chr 4 pos 12128292 (W/12128234-12128292) on the TAIR10). The sequences' 1-59 bps mapped on the genome, 60-79 bps onto the T-DNA. Pools P 11, R 13, C 19 N 14. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO361232.

SALKseq_036985.4 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G14450.1 (at chr 5 pos 4659194 (W/4659152-4659194) on the TAIR10). The sequences' 1-43 bps mapped on the genome, 60-79 bps onto the T-DNA. Pools P 11, R 13, C 19 N 14. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO364592.

Donation Date: 2002-01-02

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_036985

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_036985C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT3G48630 AT3G48640 AT4G21950 AT4G21960 AT4G23150 AT5G14450 At3g06750

EMBL accession:

BH747869

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation - At3g06750 F3E22.11 L300-3'

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.