NASC

NASC ID: N539084

Name: SALK_039084

ABRC stock number: SALK_039084

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_039084.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G62750.1 (at chr 1 pos 23233693 (W/23233636-23233693) on the TAIR10). The sequences' 1-58 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 12, R 12, C 14 N 14. The insertion site is at the 3' end of the sequence mapping. Genbank Acc KO351346.

SALKseq_039084.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G31020.1=UTR3 and AT1G31030.1 (at chr 1 pos 11061185 (C/11061185-11061263) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 12, R 12, C 14 N 14. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO344646.

SALKseq_039084.101 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G05600.1 (at chr 2 pos 2073594 (W/2073521-2073594) on the TAIR10). The sequences' 1-73 bps mapped on the genome, 61-79 bps onto the T-DNA. Pools: P 12, R 12, C 14 N 14. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO354681.

SALKseq_039084.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT1G31660.1 (at chr 1 pos 11333700 (C/11333700-11333748) on the TAIR10). The sequences' 1-49 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 12, R 12, C 14 N 14. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO347995.

Donation Date: 2002-01-01

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_039084

Part of : SALK lines

Stock type: individual line

Material type: seed

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G31020 AT1G31030 AT1G31660 AT2G05600 At1g62750

EMBL accession:

ED583988

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At1g62750

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.