NASC

NASC ID: N539755

Name: SALK_039755

ABRC stock number: SALK_039755

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_039755.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT1G63810.1 (at chr 1 pos 23676029 (C/23676029-23676093) on the TAIR10). The sequences' 1-65 bps mapped on the genome, 60-78 bps onto the T-DNA. Pools P 1, R 4, C 10 N 6. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO358051.

SALKseq_039755.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G26090.1=UTR3 and AT3G26100.2=P355 (at chr 3 pos 9536359 (W/9536304-9536359) on the TAIR10). The sequences' 1-56 bps mapped on the genome, 56-79 bps onto the T-DNA. Pools P 1, R 4, C 10 N 6. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO361400.

SALKseq_039755.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G26115.2=UTR3 and AT3G26120.1 (at chr 3 pos 9546147 (C/9546147-9546207) on the TAIR10). The sequences' 1-61 bps mapped on the genome, 58-79 bps onto the T-DNA. Pools P 1, R 4, C 10 N 6. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO364760.

Donation Date: 2002-01-02

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_039755

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_039755C.

Status	Price (£)
Available to order at NASC	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			At1g63810 AT3G26090 AT3G26100 AT3G26115 AT3G26120

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.