NASC Stock Detail Page
NASC ID: N542876
Name: SALK_042876
ABRC stock number: SALK_042876
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. Associated Polymorphisms: SALKseq_042876.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT1G73350.3 (at chr 1 pos 27577336 (W/27577280-27577336) on the TAIR10). The sequences' 1-57 bps mapped on the genome, 60-79 bps onto the T-DNA. Pools P 12, R 15, C 15 N 18. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO398345. SALKseq_042876.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G28401.1 and AT2G28405.1 (at chr 2 pos 12152926 (W/12152887-12152926) on the TAIR10). The sequences' 1-40 bps mapped on the genome, 53-79 bps onto the T-DNA. Pools P 12, R 15, C 15 N 18. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO401687. SALKseq_042876.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G03038 (around chr 4 pos 1340157 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc KO415011. SALKseq_042876.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G03050.2=UTR3 and AT4G03060 (at chr 4 pos 1351639 (W/1351597-1351639) on the TAIR10). The sequences' 1-43 bps mapped on the genome, 49-79 bps onto the T-DNA. Pools P 12, R 15, C 15 N 18. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO408357. SALKseq_042876.4 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G11800.1=P355 and AT4G11810.1=P355 (at chr 4 pos 7100945 (W/7100880-7100945) on the TAIR10). The sequences' 1-66 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 12, R 15, C 15 N 18. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO411703.
Donation Date: 2002-01-01
| Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_042876
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: Homozygous/confirmed line: SALK_042876C.
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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Phenotype
Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At1g73350
References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.