NASC

NASC ID: N543490

Name: SALK_043490

ABRC stock number: SALK_043490

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_043490.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 3' of AT5G47810.1 (at chr 5 pos 19356521 (C/19356521-19356585) on the TAIR10). The sequences' 1-65 bps mapped on the genome, 60-77 bps onto the T-DNA. Pools P 18, R 11, C 18 N 19. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO351529.

SALKseq_043490.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G70450.1 (at chr 1 pos 26552847 (C/26552847-26552925) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 18, R 11, C 18 N 19. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO411726.

SALKseq_043490.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G70460.1 (at chr 1 pos 26557297 (W/26557245-26557297) on the TAIR10). The sequences' 1-53 bps mapped on the genome, 60-79 bps onto the T-DNA. Pools P 18, R 11, C 18 N 19. The insertion site is at the 3' end of the sequence mapping. Genbank Acc KO341474.

SALKseq_043490.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G42186=P355 and AT3G42190.1=P355 (at chr 3 pos 14363510 (C/14363510-14363568) on the TAIR10). The sequences' 1-59 bps mapped on the genome, 60-79 bps onto the T-DNA. Pools P 18, R 11, C 18 N 19. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO344828.

SALKseq_043490.4 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G09110.1=P355 and AT3G09120.1=UTR3 (at chr 3 pos 2796624 (W/2796563-2796624) on the TAIR10). The sequences' 1-63 bps mapped on the genome, 57-79 bps onto the T-DNA. Pools P 18, R 11, C 18 N 19. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO348180.

Donation Date: 2002-01-02

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_043490

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_043490C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G70450 AT1G70460 AT3G09110 AT3G09120 AT3G42186 AT3G42190 At5g47810

EMBL accession:

BH748181

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation - At5g47810 MCA23.13 L300-3'

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.