NASC Stock Detail Page
NASC ID: N544020
Name: SALK_044020
ABRC stock number: SALK_044020
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. July 2002 TAIR gene annotation - At3g02600. F16B3.23 L300-5'. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2. Associated Polymorphisms: SALKseq_044020.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT3G02600.5 (at chr 3 pos 551291 (W/551240-551291) on the TAIR10). The sequences' 1-52 bps mapped on the genome, 54-76 bps onto the T-DNA. Pools P 15, R 17, C 16 N 15. The insertion site is at the 3' end of the sequence mapping. Genbank Acc KO381676. SALKseq_044020.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G24610.1 (at chr 1 pos 8722370 (W/8722310-8722370) on the TAIR10). The sequences' 1-61 bps mapped on the genome, 57-78 bps onto the T-DNA. Pools: P 15, R 17, C 16 N 15. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO378330. SALKseq_044020.101 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT3G20580.1 (at chr 3 pos 7190623 (W/7190545-7190623) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 15, R 17, C 16 N 15. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO385026. SALKseq_044020.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G12950.1=UTR3 and AT5G12960.1=P355 (at chr 5 pos 4097226 (W/4097162-4097226) on the TAIR10). The sequences' 1-65 bps mapped on the genome, 59-79 bps onto the T-DNA. Pools P 15, R 17, C 16 N 15. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO388374.
Donation Date: 2002-01-07
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_044020
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: Homozygous/confirmed line: SALK_044020C.
Status | Price (£) |
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Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.