NASC Stock Detail Page
NASC ID: N544073
Name: SALK_044073
ABRC stock number: SALK_044073
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. March 2002 TAIR gene annotation: F15H11.11 L300-3' Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At1g70885. Associated Polymorphisms: SALKseq_044073.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G70880.1=P355 and AT1G70885 (at chr 1 pos 26724726 (C/26724726-26724773) on the TAIR10). The sequences' 1-48 bps mapped on the genome, 53-79 bps onto the T-DNA. Pools P 13, R 16, C 18 N 15. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO364942. SALKseq_044073.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT1G27050.1 (at chr 1 pos 9393498 (C/9393498-9393559) on the TAIR10). The sequences' 1-62 bps mapped on the genome, 61-79 bps onto the T-DNA. Pools P 13, R 16, C 18 N 15. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO368278. SALKseq_044073.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G29630.3=UTR3 and AT2G29640.1 (at chr 2 pos 12670542 (W/12670481-12670542) on the TAIR10). The sequences' 1-62 bps mapped on the genome, 53-79 bps onto the T-DNA. Pools P 13, R 16, C 18 N 15. The insertion site is at the 3' end of the sequence mapping. Genbank Acc KO371633. SALKseq_044073.5 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G05670.1=UTR3 and AT3G05675.1=UTR3 (at chr 3 pos 1657355 (W/1657300-1657355) on the TAIR10). The sequences' 1-56 bps mapped on the genome, 57-79 bps onto the T-DNA. Pools P 13, R 16, C 18 N 15. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO374988. SALKseq_044073.6 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT3G57157 (at chr 3 pos 21156742 (W/21156684-21156742) on the TAIR10). The sequences' 1-59 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 13, R 16, C 18 N 15. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO378334.
Donation Date: 2002-03-21
| Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_044073
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: Homozygous/confirmed line: SALK_044073C.
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.