NASC

NASC ID: N546131

Name: SALK_046131

ABRC stock number: SALK_046131

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_046131.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G46790.1 (at chr 3 pos 17232103 (W/17232042-17232103) on the TAIR10). The sequences' 1-62 bps mapped on the genome, 61-79 bps onto the T-DNA. Pools P 17, R 13, C 14 N 19. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO398518.

SALKseq_046131.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G30757.1=UTR3 and AT1G30760.1=P355 (at chr 1 pos 10917857 (C/10917857-10917902) on the TAIR10). The sequences' 1-46 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 17, R 13, C 14 N 19. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO395179.

SALKseq_046131.101 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intron of AT5G45550.1 (at chr 5 pos 18463866 (C/18463866-18463902) on the TAIR10). The sequences' 1-37 bps mapped on the genome, 34-60 bps onto the T-DNA. Pools P 17, R 13, C 14 N 19. The insertion site is at the 3' end of the sequence mapping. Genbank Acc KO401865.

SALKseq_046131.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G45905 and AT5G45910.1=UTR3 (at chr 5 pos 18620270 (W/18620215-18620270) on the TAIR10). The sequences' 1-56 bps mapped on the genome, 58-79 bps onto the T-DNA. Pools P 17, R 13, C 14 N 19. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO405186.

SALKseq_046131.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G52650.1 (at chr 5 pos 21356360 (W/21356313-21356360) on the TAIR10). The sequences' 1-48 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 17, R 13, C 14 N 19. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO408527.

Donation Date: 2002-01-02

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_046131

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_046131C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G30757 AT1G30760 AT5G45550 AT5G45905 AT5G45910 AT5G52650 At3g46790

EMBL accession:

BH748538

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation - At3g46790 T6H20.180 Exon

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.