NASC Stock Detail Page
NASC ID: N546294
Name: SALK_046294
ABRC stock number: SALK_046294
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. March 2002 TAIR gene annotation: F17A9.14 R300-3' Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At3g06990. Associated Polymorphisms: SALKseq_046294.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G06990.1 and AT3G06995=P355 (at chr 3 pos 2208248 (W/2208194-2208248) on the TAIR10). The sequences' 1-55 bps mapped on the genome, 56-79 bps onto the T-DNA. Pools P 12, R 19, C 14 N 17. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO411886. SALKseq_046294.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G07680.1 (at chr 1 pos 2376747 (W/2376682-2376747) on the TAIR10). The sequences' 1-66 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 12, R 19, C 14 N 17. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO405196. SALKseq_046294.101 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G12510.1 and AT4G12520.1=UTR3 (at chr 4 pos 7419421 (W/7419343-7419421) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 12, R 19, C 14 N 17. The insertion site is at the 3' end of the sequence mapping. Genbank Acc KO341633. SALKseq_046294.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT3G59690.1 (at chr 3 pos 22048883 (C/22048883-22048944) on the TAIR10). The sequences' 1-62 bps mapped on the genome, 60-79 bps onto the T-DNA. Pools P 12, R 19, C 14 N 17. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO408536. SALKseq_046294.201 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G12520.1=P355 and AT4G12530.1=UTR3 (at chr 4 pos 7423469 (W/7423419-7423469) on the TAIR10). The sequences' 1-51 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 12, R 19, C 14 N 17. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO344986.
Donation Date: 2002-03-21
| Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_046294
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: Homozygous/confirmed line: SALK_046294C.
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.