NASC

NASC ID: N547066

Name: SALK_047066

ABRC stock number: SALK_047066

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_047066.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT4G02230.1 (at chr 4 pos 980895 (W/980831-980895) on the TAIR10). The sequences' 1-65 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 17, R 12, C 12 N 17. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO405236.

SALKseq_047066.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G20990.1 (at chr 3 pos 7359967 (W/7359936-7359967) on the TAIR10). The sequences' 1-32 bps mapped on the genome, 42-68 bps onto the T-DNA. Pools P 17, R 12, C 12 N 17. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO401914.

SALKseq_047066.101 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G14140.1 (at chr 5 pos 4563184 (W/4563133-4563184) on the TAIR10). The sequences' 1-52 bps mapped on the genome, 58-78 bps onto the T-DNA. Pools P 17, R 12, C 12 N 17. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO408575.

Donation Date: 2002-01-02

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_047066

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N664589 (SALK_047066C).

Status	Price (£)
Available to order at NASC	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT3G20990 At4g02230 AT5G14140

EMBL accession:

BH749000

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.