NASC Stock Detail Page
NASC ID: N547138
Name: SALK_047138
ABRC stock number: SALK_047138
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. Associated Polymorphisms: SALKseq_047138.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT3G24510.1 (at chr 3 pos 8931660 (C/8931660-8931717) on the TAIR10). The sequences' 1-58 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 20, R 16, C 15 N 18. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO345029. SALKseq_047138.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G33630.1 (at chr 4 pos 16158840 (W/16158778-16158840) on the TAIR10). The sequences' 1-64 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 20, R 16, C 15 N 18. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO348378. SALKseq_047138.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G04150.1 and AT5G04160.1=UTR3 (at chr 5 pos 1141903 (C/1141903-1141939) on the TAIR10). The sequences' 1-37 bps mapped on the genome, 59-79 bps onto the T-DNA. Pools P 20, R 16, C 15 N 18. The insertion site is at the 3' end of the sequence mapping. Genbank Acc KO351734. SALKseq_047138.4 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G47560.1 (at chr 5 pos 19289828 (C/19289828-19289864) on the TAIR10). The sequences' 1-37 bps mapped on the genome, 31-67 bps onto the T-DNA. Pools P 20, R 16, C 15 N 18. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO355065. SALKseq_047138.6 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G27310.1 and AT5G27320.1=P355 (at chr 5 pos 9627000 (C/9627000-9627066) on the TAIR10). The sequences' 1-67 bps mapped on the genome, 60-79 bps onto the T-DNA. Pools P 20, R 16, C 15 N 18. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO358403.
Donation Date: 2002-03-21
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_047138
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: Homozygous/confirmed line: SALK_047138C.
Status | Price (£) |
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Available to order at NASC | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
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Phenotype
Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.