NASC

NASC ID: N548894

Name: SALK_048894

ABRC stock number: SALK_048894

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

March 2002 TAIR gene annotation: F4D11.210 R300-3' Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At4g32590.

Associated Polymorphisms:

SALKseq_048894.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G32590.4=UTR3 and AT4G32600.1=P355 (at chr 4 pos 15722970 (W/15722924-15722970) on the TAIR10). The sequences' 1-48 bps mapped on the genome, 73-95 bps onto the T-DNA. Pools P 23, R 23, C 28 N 30. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO412022.

SALKseq_048894.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G25290.1 (at chr 5 pos 8779813 (W/8779729-8779813) on the TAIR10). The sequences' 1-85 bps mapped on the genome, 78-95 bps onto the T-DNA. Pools: P 23, R 23, C 28 N 30. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO341767.

Donation Date: 2002-03-21

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_048894

Part of : SALK lines

Stock type: individual line

Material type: seed

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			At4g32600 AT4G32600 AT5G25290 At4g32590

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.