NASC Stock Detail Page
NASC ID: N549735
Name: SALK_049735
ABRC stock number: SALK_049735
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. April 2002 TAIR gene annotation: F8K4.14 Exon Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At1g61950. Associated Polymorphisms: SALKseq_049735.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G61950.1 (at chr 1 pos 22900126 (W/22900097-22900126) on the TAIR10). The sequences' 1-30 bps mapped on the genome, 47-79 bps onto the T-DNA. Pools P 19, R 16, C 20 N 20. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO355201. SALKseq_049735.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G36390.2=P355 and AT1G36395.1=P355 (at chr 1 pos 13705652 (W/13705620-13705652) on the TAIR10). The sequences' 1-33 bps mapped on the genome, 36-77 bps onto the T-DNA. Pools P 19, R 16, C 20 N 20. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO351867. SALKseq_049735.101 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G26230.1 and AT5G26233.1 (at chr 5 pos 9177366 (C/9177366-9177444) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 19, R 16, C 20 N 20. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO358540.
Donation Date: 2002-04-30
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_049735
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: Homozygous/confirmed line: SALK_049735C.
Status | Price (£) |
---|---|
Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
---|---|---|---|
|
|
|
References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.