NASC

NASC ID: N550123

Name: SALK_050123

ABRC stock number: SALK_050123

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_050123.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G17060.1 (at chr 2 pos 7426442 (W/7426384-7426442) on the TAIR10). The sequences' 1-59 bps mapped on the genome, 56-78 bps onto the T-DNA. Pools P 22, R 22, C 24 N 27. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO402067.

SALKseq_050123.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G17744.1 (at chr 1 pos 6101791 (W/6101714-6101791) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools: P 22, R 22, C 24 N 27. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO398720.

SALKseq_050123.101 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G58060.1 and AT3G58070.1=UTR3 (at chr 3 pos 21504294 (W/21504217-21504294) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 22, R 22, C 24 N 27. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO405392.

Donation Date: 2002-01-02

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_050123

Part of : SALK lines

Stock type: individual line

Material type: seed

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G17744 AT3G58060 AT3G58070 At2g17060

EMBL accession:

BH751428

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation - At2g17060 F6P23.21 Exon

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.