NASC

NASC ID: N550214

Name: SALK_050214

ABRC stock number: SALK_050214

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_050214.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G05160.1 (at chr 4 pos 2665853 (W/2665787-2665853) on the TAIR10). The sequences' 1-67 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 23, R 27, C 26 N 27. The insertion site is at the 3' end of the sequence mapping. Genbank Acc KO408733.

SALKseq_050214.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G29420 and AT3G29430.1=P355 (at chr 3 pos 11310446 (W/11310371-11310446) on the TAIR10). The sequences' 1-76 bps mapped on the genome. Pools P 23, R 27, C 26 N 27. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO398724.

SALKseq_050214.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G29431.1 and AT3G29440.1 (at chr 3 pos 11315937 (C/11315937-11315993) on the TAIR10). The sequences' 2-58 bps mapped on the genome, 57-79 bps onto the T-DNA. Pools P 23, R 27, C 26 N 27. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO402071.

SALKseq_050214.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G19950.1=UTR3 and AT3G19960.2=P355 (at chr 3 pos 6947241 (W/6947164-6947241) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 23, R 27, C 26 N 27. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO405396.

Donation Date: 2002-01-02

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_050214

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock is only available on request as a homozygous/confirmed stock has replaced it. The replacement line is SALK_050214C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT3G19950 AT3G19960 AT3G29420 AT3G29430 AT3G29431 AT3G29440 At4g05160

EMBL accession:

BH751499

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation - At4g05160 C17L7.80 Exon

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.