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NASC ID: N552032

Name: SALK_052032

ABRC stock number: SALK_052032

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

March 2002 TAIR gene annotation: MEE13.23 Intron Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At5g36070.

Associated Polymorphisms:

SALKseq_052032.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G36070.1 (at chr 5 pos 14173037 (C/14173037-14173093) on the TAIR10). The sequences' 4-61 bps mapped on the genome, 55-78 bps onto the T-DNA. Pools P 21, R 28, C 22 N 22. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO402133.

SALKseq_052032.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G06940 and AT2G06950.1 (at chr 2 pos 2857194 (C/2857194-2857272) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 21, R 28, C 22 N 22. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO398787.

Donation Date: 2002-03-21

Donated by:  The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_052032

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_052032C.

Status Price (£)
Available £10.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene: Allele: Locus: AGI code:
EMBL accession:

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.