NASC

NASC ID: N554050

Name: SALK_054050

ABRC stock number: SALK_054050

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_054050.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G60835 and AT1G60840 (at chr 1 pos 22396998 (C/22396998-22397075) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 29, R 24, C 26 N 29. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO355376.

SALKseq_054050.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT1G62070.1 (at chr 1 pos 22943355 (C/22943355-22943402) on the TAIR10). The sequences' 1-48 bps mapped on the genome, 77-95 bps onto the T-DNA. Pools P 29, R 24, C 26 N 29. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO358715.

SALKseq_054050.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT2G39140.1 (at chr 2 pos 16329886 (W/16329818-16329886) on the TAIR10). The sequences' 1-69 bps mapped on the genome, 75-95 bps onto the T-DNA. Pools P 29, R 24, C 26 N 29. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO362059.

SALKseq_054050.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G21690.1=UTR3 and AT3G21700.3=P355 (at chr 3 pos 7643945 (W/7643878-7643945) on the TAIR10). The sequences' 1-68 bps mapped on the genome, 77-95 bps onto the T-DNA. Pools P 29, R 24, C 26 N 29. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO365410.

SALKseq_054050.4 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the UTR 3' of AT5G25070.1 (at chr 5 pos 8640985 (W/8640949-8640985) on the TAIR10). The sequences' 1-37 bps mapped on the genome, 74-95 bps onto the T-DNA. Pools P 29, R 24, C 26 N 29. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO368754.

Donation Date: 2002-03-21

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_054050

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_054050C.

Status	Price (£)
Available to order at NASC	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G60835 AT1G60840 AT1G62070 AT2G39140 AT3G21690 AT3G21700 AT5G25070

EMBL accession:

BH756676

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.