Home | About NASC | Address/Staff
Ask a question | Help

NASC

From January 1st, 2021, seed orders from EU customers will require a phytosanitary certificate. Please click for further information

NASC Stock Detail Page

NASC ID: N554169

Name: SALK_054169

ABRC stock number: SALK_054169

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

March 2002 TAIR gene annotation: F19B15.90 R300-3' Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At4g29060.

Associated Polymorphisms:

SALKseq_054169.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 3' of AT4G29060.1 (at chr 4 pos 14321423 (W/14321350-14321423) on the TAIR10). The sequences' 1-74 bps mapped on the genome, 77-95 bps onto the T-DNA. Pools P 26, R 28, C 22 N 22. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO358722.
SALKseq_054169.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT2G42395.1 (at chr 2 pos 17654343 (W/17654279-17654343) on the TAIR10). The sequences' 1-65 bps mapped on the genome, 62-89 bps onto the T-DNA. Pools P 26, R 28, C 22 N 22. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO355383.

Donation Date: 2002-03-21

Donated by:  The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_054169

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N664613 (SALK_054169C).

Status Price (£)
Available £10.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene: Allele: Locus: AGI code:
EMBL accession:

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.