NASC Stock Detail Page
NASC ID: N555542
Name: SALK_055542
ABRC stock number: SALK_055542
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. March 2002 TAIR gene annotation: F12P23.4 L300-5' Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At2g10450. Associated Polymorphisms: SALKseq_055542.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G10440.2 and AT2G10450.1 (at chr 2 pos 4020033 (C/4020033-4020078) on the TAIR10). The sequences' 1-46 bps mapped on the genome, 59-78 bps onto the T-DNA. Pools P 31, R 35, C 34 N 39. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO368836. SALKseq_055542.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT3G06380.1 (at chr 3 pos 1936083 (W/1936027-1936083) on the TAIR10). The sequences' 1-57 bps mapped on the genome, 60-78 bps onto the T-DNA. Pools P 31, R 35, C 34 N 39. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO372192. SALKseq_055542.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT5G59100.1 (at chr 5 pos 23862280 (C/23862280-23862331) on the TAIR10). The sequences' 1-52 bps mapped on the genome, 57-78 bps onto the T-DNA. Pools P 31, R 35, C 34 N 39. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO375546. SALKseq_055542.4 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G65166.1 and AT5G65170.1=P355 (at chr 5 pos 26037134 (C/26037134-26037212) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 31, R 35, C 34 N 39. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO378893.
Donation Date: 2002-03-21
| Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_055542
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: Homozygous/confirmed line: SALK_055542C.
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.