NASC

NASC ID: N556489

Name: SALK_056489

ABRC stock number: SALK_056489

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_056489.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT2G46550.1 (at chr 2 pos 19114369 (C/19114369-19114417) on the TAIR10). The sequences' 1-49 bps mapped on the genome, 59-78 bps onto the T-DNA. Pools P 30, R 29, C 27 N 26. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO372241.

SALKseq_056489.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G53730.1 (at chr 1 pos 20063402 (W/20063356-20063402) on the TAIR10). The sequences' 1-47 bps mapped on the genome, 50-78 bps onto the T-DNA. Pools P 30, R 29, C 27 N 26. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO365541.

SALKseq_056489.101 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G05180.1 (at chr 3 pos 1469684 (C/1469684-1469741) on the TAIR10). The sequences' 1-58 bps mapped on the genome, 57-78 bps onto the T-DNA. Pools P 30, R 29, C 27 N 26. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO375594.

SALKseq_056489.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G14850.1 (at chr 1 pos 5120317 (W/5120276-5120317) on the TAIR10). The sequences' 1-42 bps mapped on the genome, 55-78 bps onto the T-DNA. Pools P 30, R 29, C 27 N 26. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO368885.

SALKseq_056489.201 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT3G17020.1 (at chr 3 pos 5804422 (W/5804372-5804422) on the TAIR10). The sequences' 1-51 bps mapped on the genome, 59-78 bps onto the T-DNA. Pools P 30, R 29, C 27 N 26. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO378940.

Donation Date: 2002-03-21

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_056489

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_056489C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G14850 AT1G53730 At2g46550 AT3G05180 AT3G17020

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.