NASC Stock Detail Page
NASC ID: N556902
Name: SALK_056902
ABRC stock number: SALK_056902
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. March 2002 TAIR gene annotation: F21A20.10 Intron Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At5g27300. Associated Polymorphisms: SALKseq_056902.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G27300.1 (at chr 5 pos 9622625 (W/9622548-9622625) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 30, R 29, C 30 N 22. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO365561. SALKseq_056902.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT1G72855 (at chr 1 pos 27416123 (W/27416083-27416123) on the TAIR10). The sequences' 1-41 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 30, R 29, C 30 N 22. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO355525. SALKseq_056902.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT4G33670.1 (at chr 4 pos 16171668 (W/16171614-16171668) on the TAIR10). The sequences' 1-55 bps mapped on the genome, 57-78 bps onto the T-DNA. Pools P 30, R 29, C 30 N 22. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO358868. SALKseq_056902.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the UTR 3' of AT5G17030.1 (at chr 5 pos 5603133 (W/5603070-5603133) on the TAIR10). The sequences' 1-64 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 30, R 29, C 30 N 22. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO362212.
Donation Date: 2002-03-21
| Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_056902
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: Homozygous/confirmed line: SALK_056902C.
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.