NASC Stock Detail Page
NASC ID: N557141
Name: SALK_057141
ABRC stock number: SALK_057141
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. Associated Polymorphisms: SALKseq_057141.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G62020.1=P355 and AT3G62030.2 (at chr 3 pos 22972824 (C/22972824-22972884) on the TAIR10). The sequences' 1-61 bps mapped on the genome, 54-78 bps onto the T-DNA. Pools P 31, R 35, C 39 N 37. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO375625. SALKseq_057141.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G08735.1 (around chr 1 pos 2792451 on the TAIR10). The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO415339. SALKseq_057141.101 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G02900.1 (at chr 3 pos 646504 (W/646456-646504) on the TAIR10). The sequences' 1-49 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools: P 31, R 35, C 39 N 37. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO378972. SALKseq_057141.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G18170.1 (at chr 1 pos 6254563 (W/6254527-6254563) on the TAIR10). The sequences' 1-37 bps mapped on the genome, 55-78 bps onto the T-DNA. Pools P 31, R 35, C 39 N 37. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO368917. SALKseq_057141.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the UTR 3' of AT2G31360.1 (at chr 2 pos 13372042 (W/13372006-13372042) on the TAIR10). The sequences' 1-37 bps mapped on the genome, 60-78 bps onto the T-DNA. Pools P 31, R 35, C 39 N 37. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO372273.
Donation Date: 2002-04-30
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_057141
Part of : SALK lines
Stock type: individual line
Material type: seed
Status | Price (£) |
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Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
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Phenotype
Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.