NASC Stock Detail Page
NASC ID: N557158
Name: SALK_057158
ABRC stock number: SALK_057158
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. April 2002 TAIR gene annotation: F8A24.12 Exon Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At3g09830. Associated Polymorphisms: SALKseq_057158.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G07900.1 (at chr 3 pos 2522278 (C/2522278-2522309) on the TAIR10). The sequences' 1-32 bps mapped on the genome, 45-78 bps onto the T-DNA. Pools P 31, R 35, C 39 N 34. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO405718. SALKseq_057158.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G18550.1 and AT2G18560.1=P355 (at chr 2 pos 8054599 (W/8054551-8054599) on the TAIR10). The sequences' 1-49 bps mapped on the genome, 48-73 bps onto the T-DNA. Pools P 31, R 35, C 39 N 34. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO402390. SALKseq_057158.101 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT3G13845.1 (at chr 3 pos 4558575 (C/4558575-4558627) on the TAIR10). The sequences' 1-53 bps mapped on the genome, 49-74 bps onto the T-DNA. Pools P 31, R 35, C 39 N 34. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO409054. SALKseq_057158.2 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G12917=P355 and AT4G12920.1 (at chr 4 pos 7566335 (W/7566293-7566335) on the TAIR10). The sequences' 1-43 bps mapped on the genome, 58-78 bps onto the T-DNA. Pools P 31, R 35, C 39 N 34. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO412405.
Donation Date: 2002-04-30
| Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_057158
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: Homozygous/confirmed line: SALK_057158C.
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.