NASC

NASC ID: N557242

Name: SALK_057242

ABRC stock number: SALK_057242

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

April 2002 TAIR gene annotation: MDF20.14 R300-3' At5g55710 MDF20.15 L300-3' Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At5g55700.

Associated Polymorphisms:

SALKseq_057242.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 3' of AT5G55700.1 (at chr 5 pos 22554948 (W/22554869-22554948) on the TAIR10). The sequences' 1-80 bps mapped on the genome, 78-95 bps onto the T-DNA. Pools P 30, R 28, C 28 N 23. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO409057.

SALKseq_057242.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G25780.1=UTR3 and AT3G25790.1=P355 (at chr 3 pos 9410928 (W/9410855-9410928) on the TAIR10). The sequences' 1-74 bps mapped on the genome, 75-95 bps onto the T-DNA. Pools P 30, R 28, C 28 N 23. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO402393.

SALKseq_057242.2 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G51795.1 and AT5G51800.1=P355 (at chr 5 pos 21047017 (W/21046940-21047017) on the TAIR10). The sequences' 1-78 bps mapped on the genome, 75-95 bps onto the T-DNA. Pools P 30, R 28, C 28 N 23. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO405721.

Donation Date: 2002-04-30

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_057242

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_057242C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT3G25780 AT3G25790 AT5G51795 AT5G51800 At5g55700 AT5G55700 At5g55710

EMBL accession:

BH790524 ED590160

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.