NASC

NASC ID: N557690

Name: SALK_057690

ABRC stock number: SALK_057690

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

April 2002 TAIR gene annotation: MLM24.2 R300-3' Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At3g23310.

Associated Polymorphisms:

SALKseq_057690.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G23310.1=UTR3 and AT3G23320.1 (at chr 3 pos 8343734 (W/8343705-8343734) on the TAIR10). The sequences' 1-30 bps mapped on the genome, 27-69 bps onto the T-DNA. Pools P 32, R 34, C 31 N 36. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO385699.

SALKseq_057690.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G39590.1 (around chr 1 pos 14810199 on the TAIR10). The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO415350.

SALKseq_057690.101 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G30406.1 (at chr 5 pos 11371126 (C/11371126-11371186) on the TAIR10). The sequences' 1-61 bps mapped on the genome, 57-78 bps onto the T-DNA. Pools P 32, R 34, C 31 N 36. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO389046.

SALKseq_057690.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G42256.1 (around chr 3 pos 14431320 on the TAIR10). The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO415351.

SALKseq_057690.201 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G24880.1 and AT5G24890.1=UTR3 (at chr 5 pos 8554680 (W/8554615-8554680) on the TAIR10). The sequences' 2-66 bps mapped on the genome, 60-77 bps onto the T-DNA. Pools P 32, R 34, C 31 N 36. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO392381.

Donation Date: 2002-04-30

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_057690

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_057690C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G39590 AT3G23320 AT3G42256 AT5G24880 AT5G24890 AT5G30406 At3g23310

EMBL accession:

ED590395

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.