NASC

NASC ID: N558587

Name: SALK_058587

ABRC stock number: SALK_058587

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

April 2002 TAIR gene annotation: T10K17.40 Exon Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At3g57830.

Associated Polymorphisms:

SALKseq_058587.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G57830.1 (at chr 3 pos 21419850 (W/21419796-21419850) on the TAIR10). The sequences' 1-55 bps mapped on the genome, 55-78 bps onto the T-DNA. Pools P 34, R 32, C 36 N 32. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO358959.

SALKseq_058587.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT3G57860.1 (at chr 3 pos 21426422 (W/21426358-21426422) on the TAIR10). The sequences' 1-65 bps mapped on the genome, 59-78 bps onto the T-DNA. Pools P 34, R 32, C 36 N 32. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO362304.

SALKseq_058587.2 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT4G08685.1 (at chr 4 pos 5550159 (W/5550120-5550159) on the TAIR10). The sequences' 1-40 bps mapped on the genome, 54-78 bps onto the T-DNA. Pools P 34, R 32, C 36 N 32. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO365653.

Donation Date: 2002-04-30

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_058587

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_058587C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT3G57860 AT4G08685 At3g57830

EMBL accession:

ED590828

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.