NASC

NASC ID: N559235

Name: SALK_059235

ABRC stock number: SALK_059235

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

March 2002 TAIR gene annotation: T25F15.18 Intron Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At3g33056.

Associated Polymorphisms:

SALKseq_059235.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G61150.1=UTR3 and AT3G61160.1=P355 (at chr 3 pos 22635194 (C/22635194-22635243) on the TAIR10). The sequences' 1-50 bps mapped on the genome, 59-78 bps onto the T-DNA. Pools P 33, R 40, C 35 N 33. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO409164.

SALKseq_059235.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G17550.1 (at chr 3 pos 6006221 (C/6006221-6006276) on the TAIR10). The sequences' 1-56 bps mapped on the genome, 50-78 bps onto the T-DNA. Pools: P 33, R 40, C 35 N 33. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO412517.

SALKseq_059235.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G18850.1 and AT4G18860.1 (at chr 4 pos 10344410 (W/10344333-10344410) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 33, R 40, C 35 N 33. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO342259.

Donation Date: 2002-03-21

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_059235

Part of : SALK lines

Stock type: individual line

Material type: seed

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT3G17550 AT3G61150 AT3G61160 AT4G18850 AT4G18860 At3g33056

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.