NASC

NASC ID: N560834

Name: SALK_060834

ABRC stock number: SALK_060834

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

April 2002 TAIR gene annotation: F13M23.80 Intron Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At4g24940.

Associated Polymorphisms:

SALKseq_060834.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G24940.1 (at chr 4 pos 12825743 (C/12825743-12825821) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 14, R 20, C 15 N 11. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO412616.

SALKseq_060834.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G17990.1 (at chr 5 pos 5958012 (C/5958012-5958075) on the TAIR10). The sequences' 1-64 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 14, R 20, C 15 N 11. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO342358.

SALKseq_060834.4 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G28700.2=UTR3 and AT3G28705.1 (at chr 3 pos 10763561 (W/10763526-10763561) on the TAIR10). The sequences' 1-36 bps mapped on the genome, 37-79 bps onto the T-DNA. Pools P 14, R 20, C 15 N 11. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO409264.

Donation Date: 2002-04-30

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_060834

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_060834C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT3G28700 AT3G28705 AT5G17990 At4g24940

EMBL accession:

BH791683

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.