NASC Stock Detail Page
NASC ID: N561002
Name: SALK_061002
ABRC stock number: SALK_061002
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. April 2002 TAIR gene annotation: M3E9.160 Exon Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At4g26410. Associated Polymorphisms: SALKseq_061002.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G26410.1 (at chr 4 pos 13348743 (W/13348699-13348743) on the TAIR10). The sequences' 1-45 bps mapped on the genome, 56-78 bps onto the T-DNA. Pools P 34, R 40, C 39 N 38. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO365792. SALKseq_061002.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT1G05900.1 (at chr 1 pos 1786748 (W/1786704-1786748) on the TAIR10). The sequences' 1-45 bps mapped on the genome, 59-78 bps onto the T-DNA. Pools P 34, R 40, C 39 N 38. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO359095. SALKseq_061002.101 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 3' of AT5G03555.1 (at chr 5 pos 898256 (W/898204-898256) on the TAIR10). The sequences' 1-53 bps mapped on the genome, 52-78 bps onto the T-DNA. Pools P 34, R 40, C 39 N 38. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO369136. SALKseq_061002.2 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G29305.1 (at chr 3 pos 11249428 (W/11249368-11249428) on the TAIR10). The sequences' 1-62 bps mapped on the genome, 57-78 bps onto the T-DNA. Pools: P 34, R 40, C 39 N 38. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO362442.
Donation Date: 2002-04-30
| Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_061002
Part of : SALK lines
Stock type: individual line
Material type: seed
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.