NASC Stock Detail Page
NASC ID: N561042
Name: SALK_061042
ABRC stock number: SALK_061042
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. April 2002 TAIR gene annotation: MKP11.3 R300-3' Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At5g17180. Associated Polymorphisms: SALKseq_061042.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G63590.1 (at chr 1 pos 23580223 (W/23580173-23580223) on the TAIR10). The sequences' 1-51 bps mapped on the genome, 57-76 bps onto the T-DNA. Pools P 36, R 35, C 39 N 32. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO342370. SALKseq_061042.001 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT5G17190.1 (at chr 5 pos 5651939 (W/5651895-5651939) on the TAIR10). The sequences' 1-45 bps mapped on the genome, 60-78 bps onto the T-DNA. Pools P 36, R 35, C 39 N 32. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO352426. SALKseq_061042.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G18870.1 (at chr 1 pos 6516153 (C/6516153-6516213) on the TAIR10). The sequences' 1-61 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 36, R 35, C 39 N 32. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO345725. SALKseq_061042.2 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G18871.1 (at chr 1 pos 6519402 (W/6519325-6519402) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 36, R 35, C 39 N 32. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO349069.
Donation Date: 2002-04-30
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_061042
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: Homozygous/confirmed line: SALK_061042C.
Status | Price (£) |
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Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.