NASC

NASC ID: N561502

Name: SALK_061502

ABRC stock number: SALK_061502

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

April 2002 TAIR gene annotation: T25N20.5 R300-3' At1g05410 T25N20.6 L300-3' Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At1g05400.

Associated Polymorphisms:

SALKseq_061502.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 3' of AT1G05400.1 (at chr 1 pos 1585425 (W/1585368-1585425) on the TAIR10). The sequences' 1-58 bps mapped on the genome, 60-78 bps onto the T-DNA. Pools P 35, R 39, C 39 N 37. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO342399.

SALKseq_061502.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G23850 (at chr 2 pos 10156276 (W/10156210-10156276) on the TAIR10). The sequences' 1-67 bps mapped on the genome, 58-78 bps onto the T-DNA. Pools P 35, R 39, C 39 N 37. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO345754.

SALKseq_061502.2 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G35495.1 (at chr 5 pos 13695755 (W/13695691-13695755) on the TAIR10). The sequences' 1-65 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 35, R 39, C 39 N 37. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO349098.

Donation Date: 2002-04-30

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_061502

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_061502C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G05400 AT2G23850 AT5G35495 At1g05410

EMBL accession:

BH791830 ED591949

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.