NASC Stock Detail Page
NASC ID: N562132
Name: SALK_062132
ABRC stock number: SALK_062132
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. February 2003 TAIR gene annotation - At4g39990 T5J17.1 60 R300-3' At4g40000 T5J17.170 L300-3' T5J17.1 60 R300-3' At4g40000 T5J17.170 L300-3'. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2. Associated Polymorphisms: SALKseq_062132.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G39990.1 (at chr 4 pos 18543701 (W/18543647-18543701) on the TAIR10). The sequences' 1-55 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 37, R 33, C 35 N 39. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO365864. SALKseq_062132.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G17080.1=P355 and AT1G17090.1=UTR3 (at chr 1 pos 5842183 (C/5842183-5842243) on the TAIR10). The sequences' 1-61 bps mapped on the genome, 56-78 bps onto the T-DNA. Pools P 37, R 33, C 35 N 39. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO355824. SALKseq_062132.101 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G37220.1 and AT5G37230.1 (at chr 5 pos 14735044 (C/14735044-14735103) on the TAIR10). The sequences' 1-60 bps mapped on the genome, 58-78 bps onto the T-DNA. Pools P 37, R 33, C 35 N 39. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO369207. SALKseq_062132.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G46060.1 (at chr 2 pos 18944912 (W/18944835-18944912) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 37, R 33, C 35 N 39. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO359167. SALKseq_062132.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G46780.1=UTR3 and AT2G46790.1=P355 (at chr 2 pos 19231683 (W/19231635-19231683) on the TAIR10). The sequences' 1-49 bps mapped on the genome, 47-68 bps onto the T-DNA. Pools P 37, R 33, C 35 N 39. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO362514.
Donation Date: 2003-03-11
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_062132
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: Homozygous/confirmed line: SALK_062132C.
Status | Price (£) |
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Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.