NASC Stock Detail Page
NASC ID: N562340
Name: SALK_062340
ABRC stock number: SALK_062340
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. April 2002 TAIR gene annotation: T11H3.110 R300-5' Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At5g25100. Associated Polymorphisms: SALKseq_062340.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT5G25100.1 (at chr 5 pos 8651432 (C/8651432-8651478) on the TAIR10). The sequences' 1-47 bps mapped on the genome, 58-78 bps onto the T-DNA. Pools P 37, R 31, C 36 N 32. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO402692. SALKseq_062340.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G10300.1 (at chr 2 pos 3961922 (W/3961866-3961922) on the TAIR10). The sequences' 1-57 bps mapped on the genome, 58-78 bps onto the T-DNA. Pools P 37, R 31, C 36 N 32. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO389318. SALKseq_062340.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G10450.1 and AT2G10460.1=P355 (at chr 2 pos 4021068 (C/4021068-4021123) on the TAIR10). The sequences' 1-56 bps mapped on the genome, 57-78 bps onto the T-DNA. Pools P 37, R 31, C 36 N 32. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO392657. SALKseq_062340.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G26940.1 (at chr 3 pos 9938670 (C/9938670-9938732) on the TAIR10). The sequences' 1-63 bps mapped on the genome, 56-78 bps onto the T-DNA. Pools P 37, R 31, C 36 N 32. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO396007. SALKseq_062340.4 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G59420.1=UTR3 and AT5G59430.4=P355 (at chr 5 pos 23966136 (W/23966096-23966136) on the TAIR10). The sequences' 1-41 bps mapped on the genome, 60-78 bps onto the T-DNA. Pools P 37, R 31, C 36 N 32. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO399344.
Donation Date: 2002-04-30
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_062340
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: Homozygous/confirmed line: SALK_062340C.
Status | Price (£) |
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Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.