NASC

NASC ID: N563231

Name: SALK_063231

ABRC stock number: SALK_063231

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

April 2002 TAIR gene annotation: F2D10.28 L300-3' Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At1g20790.

Associated Polymorphisms:

SALKseq_063231.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 3' of AT1G20780.1 (at chr 1 pos 7220792 (W/7220734-7220792) on the TAIR10). The sequences' 1-59 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 39, R 35, C 39 N 37. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO412758.

SALKseq_063231.001 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G20780.1=UTR3 and AT1G20790.1 (at chr 1 pos 7220831 (C/7220831-7220906) on the TAIR10). The sequences' 1-77 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 39, R 35, C 39 N 37. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO342500.

SALKseq_063231.2 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G53560.1=P355 and AT1G53570.1=P355 (at chr 1 pos 19986843 (W/19986789-19986843) on the TAIR10). The sequences' 1-55 bps mapped on the genome, 54-78 bps onto the T-DNA. Pools P 39, R 35, C 39 N 37. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO409406.

Donation Date: 2002-04-30

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_063231

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_063231C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G20780 AT1G53560 AT1G53570 At1g20790

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.