NASC

NASC ID: N563820

Name: SALK_063820

ABRC stock number: SALK_063820

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

April 2002 TAIR gene annotation: F28K19.14 Intron Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At1g77930.

Associated Polymorphisms:

SALKseq_063820.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G77930.1 (at chr 1 pos 29301778 (W/29301717-29301778) on the TAIR10). The sequences' 1-62 bps mapped on the genome, 56-78 bps onto the T-DNA. Pools P 38, R 40, C 37 N 34. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO406101.

SALKseq_063820.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G67328=P355 and AT1G67340.1=P355 (at chr 1 pos 25223263 (C/25223263-25223341) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 38, R 40, C 37 N 34. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO402775.

SALKseq_063820.101 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G03450.1 (at chr 2 pos 1043203 (W/1043138-1043203) on the TAIR10). The sequences' 1-66 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 38, R 40, C 37 N 34. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO409442.

SALKseq_063820.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G02650.1 and AT2G02660.1 (at chr 2 pos 737262 (C/737262-737319) on the TAIR10). The sequences' 1-58 bps mapped on the genome, 61-79 bps onto the T-DNA. Pools P 38, R 40, C 37 N 34. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO412795.

SALKseq_063820.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G59760.1 (at chr 5 pos 24076486 (W/24076426-24076486) on the TAIR10). The sequences' 1-61 bps mapped on the genome, 60-79 bps onto the T-DNA. Pools P 38, R 40, C 37 N 34. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO342538.

Donation Date: 2002-04-30

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_063820

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_063820C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G67328 AT1G67340 AT2G02650 AT2G02660 AT2G03450 AT5G59760 At1g77930

EMBL accession:

ED594185

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.