NASC Stock Detail Page
NASC ID: N564609
Name: SALK_064609
ABRC stock number: SALK_064609
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. Associated Polymorphisms: SALKseq_064609.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT3G60420.1 (at chr 3 pos 22334209 (C/22334209-22334264) on the TAIR10). The sequences' 1-56 bps mapped on the genome, 56-76 bps onto the T-DNA. Pools P 41, R 45, C 44 N 47. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO412845. SALKseq_064609.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G65490.1 (at chr 5 pos 26180341 (W/26180281-26180341) on the TAIR10). The sequences' 1-61 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools: P 41, R 45, C 44 N 47. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO342588. SALKseq_064609.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT5G02502.1 (at chr 5 pos 559260 (W/559221-559260) on the TAIR10). The sequences' 1-40 bps mapped on the genome, 72-95 bps onto the T-DNA. Pools P 41, R 45, C 44 N 47. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO345941.
Donation Date: 2002-05-31
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_064609
Part of : SALK lines
Stock type: individual line
Material type: seed
Status | Price (£) |
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Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
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Phenotype
Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.