NASC

NASC ID: N564909

Name: SALK_064909

ABRC stock number: SALK_064909

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

May TAIR gene annotation Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At1g43580.

Associated Polymorphisms:

SALKseq_064909.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G43580.1 (at chr 1 pos 16410762 (W/16410709-16410762) on the TAIR10). The sequences' 1-54 bps mapped on the genome, 44-78 bps onto the T-DNA. Pools P 40, R 40, C 32 N 35. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO369368.

SALKseq_064909.001 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT2G32415.1 (at chr 2 pos 13765645 (W/13765606-13765645) on the TAIR10). The sequences' 1-40 bps mapped on the genome, 45-78 bps onto the T-DNA. Pools P 40, R 40, C 32 N 35. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO376083.

SALKseq_064909.1 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G61490.1 (at chr 1 pos 22685463 (W/22685407-22685463) on the TAIR10). The sequences' 1-57 bps mapped on the genome, 46-78 bps onto the T-DNA. Pools P 40, R 40, C 32 N 35. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO372725.

Donation Date: 2002-05-31

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_064909

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock is only available on request as a homozygous/confirmed stock has replaced it. The replacement line is SALK_064909C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G61490 AT2G32415 At1g43580

EMBL accession:

BH792697

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.