NASC Stock Detail Page
NASC ID: N565277
Name: SALK_065277
ABRC stock number: SALK_065277
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. May TAIR gene annotation Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At1g11180. Associated Polymorphisms: SALKseq_065277.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT1G11180.1 (at chr 1 pos 3745815 (W/3745754-3745815) on the TAIR10). The sequences' 1-62 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 38, R 31, C 35 N 40. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO342630. SALKseq_065277.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT1G51805.1 (at chr 1 pos 19225832 (C/19225832-19225884) on the TAIR10). The sequences' 1-53 bps mapped on the genome, 56-78 bps onto the T-DNA. Pools P 38, R 31, C 35 N 40. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO412886. SALKseq_065277.101 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G27330.1=P355 and AT4G27340.1=UTR3 (at chr 4 pos 13685699 (W/13685662-13685699) on the TAIR10). The sequences' 1-38 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 38, R 31, C 35 N 40. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO345982. SALKseq_065277.2 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G50140.1 and AT5G50150.1=UTR3 (at chr 5 pos 20400335 (W/20400275-20400335) on the TAIR10). The sequences' 1-61 bps mapped on the genome, 57-78 bps onto the T-DNA. Pools P 38, R 31, C 35 N 40. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO349324.
Donation Date: 2002-05-31
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_065277
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: Homozygous/confirmed line: SALK_065277C.
Status | Price (£) |
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Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.