NASC

NASC ID: N566526

Name: SALK_066526

ABRC stock number: SALK_066526

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_066526.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G55160.1 and AT3G55170.1=UTR3 (at chr 3 pos 20452782 (W/20452712-20452782) on the TAIR10). The sequences' 1-71 bps mapped on the genome, 76-95 bps onto the T-DNA. Pools P 42, R 45, C 49 N 47. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO426629.

SALKseq_066526.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G50520.1=UTR3 and AT3G50530.1=P355 (at chr 3 pos 18752305 (W/18752227-18752305) on the TAIR10). The sequences' 1-79 bps mapped on the genome, 72-95 bps onto the T-DNA. Pools P 42, R 45, C 49 N 47. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO423312.

SALKseq_066526.2 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT4G15910.1 (at chr 4 pos 9028251 (C/9028251-9028302) on the TAIR10). The sequences' 1-52 bps mapped on the genome, 57-84 bps onto the T-DNA. Pools P 42, R 45, C 49 N 47. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO429943.

Donation Date: 2003-03-11

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_066526

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_066526C.

Status	Price (£)
Available to order at NASC	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT3G50520 AT3G50530 AT3G55160 AT3G55170 AT4G15910

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.