NASC

NASC ID: N566934

Name: SALK_066934

ABRC stock number: SALK_066934

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_066934.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G23610.1 (at chr 2 pos 10045571 (C/10045571-10045644) on the TAIR10). The sequences' 1-74 bps mapped on the genome, 76-95 bps onto the T-DNA. Pools: P 43, R 50, C 42 N 47. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO382897.

SALKseq_066934.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G50060.1=P355 and AT3G50070.1=UTR3 (at chr 3 pos 18560559 (W/18560493-18560559) on the TAIR10). The sequences' 1-67 bps mapped on the genome, 78-95 bps onto the T-DNA. Pools P 43, R 50, C 42 N 47. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO386246.

SALKseq_066934.4 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G06778.1=P355 and AT3G06780.1=P355 (at chr 3 pos 2141895 (C/2141895-2141955) on the TAIR10). The sequences' 1-61 bps mapped on the genome, 77-95 bps onto the T-DNA. Pools P 43, R 50, C 42 N 47. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO389593.

SALKseq_066934.5 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT4G37880.1 (at chr 4 pos 17809715 (W/17809641-17809715) on the TAIR10). The sequences' 1-75 bps mapped on the genome, 77-95 bps onto the T-DNA. Pools P 43, R 50, C 42 N 47. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO392936.

Donation Date: 2002-05-31

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_066934

Part of : SALK lines

Stock type: individual line

Material type: seed

Status	Price (£)
Available to order at NASC	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT2G23610 AT3G06778 AT3G06780 AT3G50060 AT3G50070 AT4G37880

EMBL accession:

ED596007

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.