NASC Stock Detail Page
NASC ID: N567323
Name: SALK_067323
ABRC stock number: SALK_067323
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. June 2002 TAIR gene annotation . Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2. Associated Polymorphisms: SALKseq_067323.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G27870.1 (at chr 1 pos 9707004 (C/9707004-9707081) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools: P 42, R 48, C 48 N 48. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO402985. SALKseq_067323.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G37460.1=P355 and AT2G37470.1=P355 (at chr 2 pos 15732444 (C/15732444-15732521) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 42, R 48, C 48 N 48. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO406314. SALKseq_067323.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G06142.1 (at chr 3 pos 1859475 (C/1859475-1859539) on the TAIR10). The sequences' 1-65 bps mapped on the genome, 60-78 bps onto the T-DNA. Pools: P 42, R 48, C 48 N 48. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO409652. SALKseq_067323.4 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G54280.1 (at chr 3 pos 20098250 (W/20098199-20098250) on the TAIR10). The sequences' 1-52 bps mapped on the genome, 57-78 bps onto the T-DNA. Pools P 42, R 48, C 48 N 48. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO413008. SALKseq_067323.5 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G55730.1=P355 and AT3G55734=P355 (at chr 3 pos 20685644 (C/20685644-20685704) on the TAIR10). The sequences' 1-61 bps mapped on the genome, 57-78 bps onto the T-DNA. Pools P 42, R 48, C 48 N 48. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO342751.
Donation Date: 2002-01-06
| Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_067323
Part of : SALK lines
Stock type: individual line
Material type: seed
| Status | Price (£) |
|---|---|
| Available to order at NASC | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.