NASC Stock Detail Page
NASC ID: N567905
Name: SALK_067905
ABRC stock number: SALK_067905
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. Associated Polymorphisms: SALKseq_067905.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G21400.1 (at chr 4 pos 11401320 (W/11401240-11401320) on the TAIR10). The sequences' 1-81 bps mapped on the genome, 78-95 bps onto the T-DNA. Pools: P 43, R 41, C 41 N 46. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO379608. SALKseq_067905.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G16855.1 and AT4G16870.1 (at chr 4 pos 9483750 (W/9483715-9483750) on the TAIR10). The sequences' 1-36 bps mapped on the genome. Pools P 43, R 41, C 41 N 46. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO382955. SALKseq_067905.4 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G16920.1 (at chr 4 pos 9520117 (C/9520117-9520211) on the TAIR10). The sequences' 1-95 bps mapped on the genome. Pools P 43, R 41, C 41 N 46. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO386301. SALKseq_067905.5 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G16950.1 (at chr 4 pos 9540049 (W/9539995-9540049) on the TAIR10). The sequences' 25-79 bps mapped on the genome, 77-95 bps onto the T-DNA. Pools P 43, R 41, C 41 N 46. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO389647.
Donation Date: 2002-01-10
| Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_067905
Part of : SALK lines
Stock type: individual line
Material type: seed
| Status | Price (£) |
|---|---|
| Available to order at NASC | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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Phenotype
Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.