NASC

NASC ID: N568285

Name: SALK_068285

ABRC stock number: SALK_068285

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

December 2002 TAIR gene annotation - At3g17540 MKP6.9 L300-5' Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_068285.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G17530.1=UTR3 and AT3G17540.1 (at chr 3 pos 6002495 (C/6002495-6002533) on the TAIR10). The sequences' 1-39 bps mapped on the genome, 56-78 bps onto the T-DNA. Pools P 77, R 77, C 80 N 71. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO366232.

SALKseq_068285.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G44130.1 (at chr 2 pos 18254956 (C/18254956-18255012) on the TAIR10). The sequences' 1-57 bps mapped on the genome, 54-78 bps onto the T-DNA. Pools: P 77, R 77, C 80 N 71. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO359536.

SALKseq_068285.2 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the UTR 3' of AT3G17440.1 (at chr 3 pos 5969595 (C/5969595-5969656) on the TAIR10). The sequences' 1-62 bps mapped on the genome, 60-78 bps onto the T-DNA. Pools P 77, R 77, C 80 N 71. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO362888.

Donation Date: 2003-01-30

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_068285

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_068285C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT2G44130 AT3G17440 AT3G17530 At3g17540

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.