NASC

NASC ID: N569238

Name: SALK_069238

ABRC stock number: SALK_069238

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

May TAIR gene annotation Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At1g33110.

Associated Polymorphisms:

SALKseq_069238.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intron of AT1G33110.1 (at chr 1 pos 12006542 (W/12006497-12006542) on the TAIR10). The sequences' 1-46 bps mapped on the genome, 59-78 bps onto the T-DNA. Pools P 46, R 42, C 48 N 45. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO369634.

SALKseq_069238.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G78815.1=P355 and AT1G78820.1=UTR3 (at chr 1 pos 29633757 (W/29633694-29633757) on the TAIR10). The sequences' 1-64 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 46, R 42, C 48 N 45. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO372995.

SALKseq_069238.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G24761 and AT2G24762.1=P355 (at chr 2 pos 10554381 (W/10554304-10554381) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 46, R 42, C 48 N 45. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO376344.

SALKseq_069238.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G13980.1 and AT2G13990.1 (at chr 2 pos 5873067 (C/5873067-5873101) on the TAIR10). The sequences' 2-36 bps mapped on the genome. Pools P 46, R 42, C 48 N 45. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO379691.

Donation Date: 2002-05-31

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_069238

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N662853 (SALK_069238C).

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G78815 AT1G78820 AT2G13980 AT2G13990 AT2G24761 AT2G24762 At1g33110

EMBL accession:

BH849085

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.