NASC

NASC ID: N570585

Name: SALK_070585

ABRC stock number: SALK_070585

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

June 2002 TAIR gene annotation - F6N18.12 R300-3' At1g32750 F6N18.13 L300-3'. At1g32740. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_070585.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 3' of AT1G32740.1 (at chr 1 pos 11846157 (W/11846113-11846157) on the TAIR10). The sequences' 1-45 bps mapped on the genome, 51-78 bps onto the T-DNA. Pools P 47, R 48, C 50 N 44. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO389808.

SALKseq_070585.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G51160.1=P355 and AT3G51171.1=P355 (at chr 3 pos 19009827 (W/19009782-19009827) on the TAIR10). The sequences' 1-45 bps mapped on the genome, 52-78 bps onto the T-DNA. Pools P 47, R 48, C 50 N 44. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO393161.

SALKseq_070585.2 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G20060.1 (at chr 5 pos 6778568 (W/6778509-6778568) on the TAIR10). The sequences' 1-59 bps mapped on the genome, 56-75 bps onto the T-DNA. Pools P 47, R 48, C 50 N 44. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO396502.

Donation Date: 2002-01-06

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_070585

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_070585C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			At1g32740 AT1G32740 AT3G51160 AT3G51171 AT5G20060 At1g32750

EMBL accession:

BH849943

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.