NASC

NASC ID: N571036

Name: SALK_071036

ABRC stock number: SALK_071036

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

June 2002 TAIR gene annotation - F14N23.17 Intron. At1g10290. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_071036.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G10290.1 (at chr 1 pos 3373019 (W/3372963-3373019) on the TAIR10). The sequences' 1-57 bps mapped on the genome, 53-78 bps onto the T-DNA. Pools P 47, R 46, C 44 N 42. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO403215.

SALKseq_071036.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G77630.1=UTR3 and AT1G77640.1=P355 (at chr 1 pos 29176140 (C/29176140-29176188) on the TAIR10). The sequences' 1-49 bps mapped on the genome, 53-78 bps onto the T-DNA. Pools P 47, R 46, C 44 N 42. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO399871.

SALKseq_071036.101 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G12780.1=P355 and AT1G12790.1=UTR3 (at chr 1 pos 4359097 (W/4359051-4359097) on the TAIR10). The sequences' 1-47 bps mapped on the genome, 44-76 bps onto the T-DNA. Pools P 47, R 46, C 44 N 42. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO406543.

SALKseq_071036.2 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G52760.1=UTR3 and AT5G52770.1 (at chr 5 pos 21389209 (W/21389167-21389209) on the TAIR10). The sequences' 1-43 bps mapped on the genome, 60-78 bps onto the T-DNA. Pools P 47, R 46, C 44 N 42. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO409879.

Donation Date: 2002-01-06

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_071036

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_071036C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G12780 AT1G12790 AT1G77630 AT1G77640 AT5G52760 AT5G52770 At1g10290

EMBL accession:

BH850268

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.