NASC Stock Detail Page
NASC ID: N571455
Name: SALK_071455
ABRC stock number: SALK_071455
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. Associated Polymorphisms: SALKseq_071455.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G30510 and AT3G30520.1 (around chr 3 pos 12128988 on the TAIR10). The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO415888. SALKseq_071455.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G02770.1=P355 and AT4G02780.1=UTR3 (around chr 4 pos 1231021 on the TAIR10). The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO415889. SALKseq_071455.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G10920.2=P355 and AT4G10925.3=UTR3 (around chr 4 pos 6700821 on the TAIR10). The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO415890. SALKseq_071455.4 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G65230.1=UTR3 and AT5G65240.2=UTR3 (at chr 5 pos 26070640 (W/26070591-26070640) on the TAIR10). The sequences' 1-50 bps mapped on the genome, 55-78 bps onto the T-DNA. Pools P 48, R 46, C 43 N 46. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO369768. SALKseq_071455.5 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G27500 and AT5G27505.1 (around chr 5 pos 9710191 on the TAIR10). The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO415891.
Donation Date: 2002-01-07
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_071455
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: Homozygous/confirmed line: SALK_071455C.
Status | Price (£) |
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Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
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Phenotype
Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.