NASC

NASC ID: N571495

Name: SALK_071495

ABRC stock number: SALK_071495

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_071495.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT5G53080.1 (at chr 5 pos 21520568 (W/21520497-21520568) on the TAIR10). The sequences' 1-72 bps mapped on the genome, 75-95 bps onto the T-DNA. Pools P 49, R 41, C 46 N 47. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO359732.

SALKseq_071495.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G35535 and AT1G35537.1 (at chr 1 pos 13103305 (W/13103260-13103305) on the TAIR10). The sequences' 1-45 bps mapped on the genome, 46-72 bps onto the T-DNA. Pools P 49, R 41, C 46 N 47. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO349705.

SALKseq_071495.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G60740.1 (at chr 1 pos 22361613 (C/22361613-22361648) on the TAIR10). The sequences' 1-36 bps mapped on the genome. Pools P 49, R 41, C 46 N 47. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO353060.

SALKseq_071495.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G30040.1=UTR3 and AT2G30050.1=P355 (at chr 2 pos 12823414 (W/12823385-12823414) on the TAIR10). The sequences' 1-30 bps mapped on the genome, 56-76 bps onto the T-DNA. Pools P 49, R 41, C 46 N 47. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO356384.

Donation Date: 2002-01-07

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_071495

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_071495C.

Status	Price (£)
Available to order at NASC	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G35535 AT1G35537 AT1G60740 AT2G30040 AT2G30050 At5g53080

EMBL accession:

CC179598

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.